Aim and Background: Thermostable DNA polymerase enzyme has been taken much into consideration due to its application in PCR and molecular biology researches and it has doubled the importance of the study on the various thermostable DNA polymerase. The purpose of this study was to assay the function and efficient production of the cold Sensitive thermostable DNA polymerase and its quick and cheap purification.Material and Methods: After synthesis of the designed gene artificially, it was cloned into the pET28 vector and then was transferred into E.coli. IPTG was used as an inducer in the study of gene expression. Initial purification of the enzyme was performed by heat treatment at 72oC and precipitation of other insoluble proteins by centrifuge. The DNA synthesis activity of crude extract was compared with commercial enzyme.Result: Recombinant cold Sensitive and thermostable Taq DNA polymerase expressed in E.coli showed a significant advantage and more desirable functionality such as activity and thermostable compared to commercial enzyme.Conclusion: With regard to importance and application level of thermostable DNA polymerase in molecular biology, the production method used in this study is practical and cost effective. Additionally, the simplicity of producing method of this enzyme and its accuracy is a good reason for artificial and local production of highly pure cold Sensitive Taq DNA polymerase.

Electric springs (ES) are often known to be demand-side power management systems in decentralized grids with renewable energy sources, which, due to their nature, inject unreliable electricity power into the system. The second type of electrical spring (ES_2) produces an intelligent Load by placing it next to a Sensitive Load, which, in addition to regulating the voltage level, will optimize various parameters of the power quality for the Sensitive Load. In imbalance grids with harmonic voltages and local non-linear Load, where conventional controllers are used, the intelligent Load cannot improve the power quality for a Sensitive Load. In this paper, a proportional resonance (PR) controller is used to regulate both the voltage level and the source voltage imbalances due to the least tracking error in the sinusoidal mode as well as a repetitive controller (RC) is designed to reduce THD and improve the power factor thanks to its infinite poles on the imaginary axis. Finally, these two suggested controllers were tested simultaneously in three-phase and single-phase grids by mathematical and machine simulation. The grid considered in this paper has harmonics up to order 17 and voltage fluctuations in the range of 0. 954 to 1. 1 per unit, as well as a local non-linear Load with 100% of the Sensitive Load size and, in the three-phase mode, the grid, has unbalanced three-phase Load. The results of the MATLAB simulation for electrical spring, controlled by the PR RC controller, which is a combination of two proportional resonance controllers and a repetitive controller in both three and single-phase mode Note that the Sensitive Load voltage is completely adjusted to its reference value, THD reduces by 80. 31% compared to the uncompensated condition and will be less than the value defined by the IEEE standard and the power factor rises up to near the unit.

Background and Aim: It is believed that a period of healing, approximately 3-4 months for mandible and 4-6 months for maxilla, are required to ensure osseointegration. Recently, a new method called Immediate Loading of implants have been proposed. This method provides improved stability of the implant by the immediate support of an iterim prosthesis. The aim of this study was to evaluate the success rate of the Immediate Loading single OPILS dental implants.Materials and Methods: Thirty-nine patients, including 26 females and 13 males, at the minimum age of 18 and maximum age of 54 years old, received 48 OPILS dental implants. In this prospective study, all samples were non-smoking, without any systemic disease or psychological problem. In all cases, and acrylic prefabricated crown was placed immediately after implant surgery. The definitive metal-ceramic crown was placed 2.5 months later in mandible and 5.5 months later in maxillae. Lack of pain, pus, implant immobility and less than 1mm bone loss were criteria of success.Results: The oral hygiene of females were better than males, 26 women had plaque index (0) and 11 of them had plaque index (1) where as out of 13 male subjects, 4 had plaque index (0), 6 had plaque index (2) and (3) had plaque index (3). The mean vertical bone loss, on mesial and distal parts, were less than 1mm, with the min. of 0 and max. of 0.4mm and the average of 0.2mm. All implants pass the defined criteria of success.Conclusion: On the basis of this short-term study, in single tooth cases, OPILS implants of Neo-Dent system can be Loaded immediately.

Background and Objectives: Diabetic Retinopathy (DR) is a vascular complication of type 1 and type 2 diabetes mellitus. The aim of this study was to determine the effects of Calcium Dobesilate (CD) on decreasing Endothelin-1 (ET-1) and high sensitivity C-Reactive Protein (hs-CRP) serum levels in patients with diabetic retinopathy.Materials and Methods: Ninety adults with type II diabetes having DR complication were included in this double-blind randomized clinical trial study. Patients categorized in two groups of case (diabetic retinopathy patents treated with CD) and control (diabetic retinopathy patents without CD treatment), age and sex-matched randomly between two groups during seventeen month period in Tabriz Nikookari eye Hospital and Sheikholrais clinical center. The case group received 500mg of CD for three month, The serum level of hs-CRP and ET-1, was recorded in all patients before and after administration of CD and compared between both groups.Results: Ninety patients with diabetic retinopathy, including of 30 males and 60 females, with the mean age of 59.18±7.13 years were enrolled in the study. The mean level of serum endothelin-1 was significantly different in the case group than control group after treatment with CD as well (0.69±0.32 pg/ml vs.0.86±0.30 pg/ml; p=0.005), and also there was a significant different of mean serum level of hs-CRP after treatment with Doxium between the case and control group, as well (3.28±3.28 mg/L vs.4.67±3.75 mg/L; p=0.006; respectively).Conclusions: Treatment with CD can decrease the serum levels of ET-1 and hs-CRP as two important inflammatory biomarkers in patients with diabetic retinopathy. More studies are recommended to be carried out in this regard.

One of the obvious reasons for most disorders in network service provisioning is network path congestion. Congestion avoidance in today's networks is too costly and sometimes impossible. With the introduction of SDN, centralizing the equipment's control plane has become possible. This paper presents an enhanced method named ESV-DBRA to avoid congestion in multi-tenant SDN networks. At first, ESV-DBRA monitors the traffic Load and delay of all network paths for each tenant individually. Then, by merging the parameters obtained from the monitoring, the Service Level Agreements (SLA), and a novel proposed cost function, it calculates the cost of the network paths per tenant. As a result, traffic for each tenant is routed through the path/paths at the lowest possible cost from the tenant's perspective. Next, the bandwidth quotas will be calculated and assigned to the tenants over their optimal routes. Afterward, whenever congestion is likely to occur in a path, ESV-DBRA automatically changes the route or bandwidth of the tenants' traffic related to this path to avoid congestion. Related algorithms are also proposed.Eventually, simulations show that the proposed method effectively increases bandwidth utilization by 10.76%.

The purpose of the present study was to investigate the effect of generative learning teaching based to pattern cognitive Load on the memorization math of 7th grade students of in the academic year of 2021- 2022. The research method is quasi-experimental with pre-test and post-test with four groups: two control group and two experimental groups. The statistical population of the study consisted of all 7th grade male and female students of District 2 of Mashhad city that 112 person sample was selected by single stage cluster sampling method. The subjects in the experimental group involved in generative learning based to cognitive Load in 16 sessions and the control group did not receive any interventions. The research instrument consisted of a 20-questions researcher-made test. To analyze the obtained data, Multivariate analysis of covariance and SPSS version 24 software were used. The results showed that generative learning teaching based to approach cognitive Load have a positive and significant effect on 7th grade students' memorization math. Based on the findings, it can be concluded that one of the effective training on math memorization students is the use of generative learning on the based Load cognitive pattern.

Background and aim: Results of Implant therapy have always been evaluated on the basis of radiographic and clinical assessments such as percussion, focused only on determination of peri-implant bone resorption and implant failures. These measurements are qualitative only and could not be considered as quantitative and reliable scales for the evaluation of stability; So they could not be statistically analysed. These difficulties could be overcome by using Resonance Frequency Analysis (RFA).RFA could be considered as a proper comparable measurement in evaluation of implant stability. This study aimed to evaluate and determine the stability of over denture Loaded implants in a number of private clinics during 2006 -2007.Materials and Methods: This cross – sectional study was conducted on 13 patients provided with Swiss plus implant Therapy Loaded by over dentures for the first step, each patient was evaluated regarding Implant Quality health Scale and put into one of five groups. Abutments were then removed and fixtures were evaluated by Osstell Mentor Instrument following the attachment of Smart peg to fixtures, Implant Stability Qoutient was then determined. Sampling was done in a sequential approach and obtained data were statistically analyzed using X2 and t-test.Results: Fourty three implants were evaluated in 13 patients, 21 in maxilla and 22 in mandible the mean I.S.Q.Equaled 70.36 ± 4.43 in maxilla and 79.1 ± 3.51 in mandible. The total mean ISQ (bimaxillary mean ISQ) equaled 84.36 ± 5.94.Conclusion: Regarding the obtained results, Over denture Loaded implants are absolutely successful treatment plans in mandible, according to their higher ISQ compared to maxilla. It is also demonstrated that stability of implants at each step of treatment Procedure is influenced by primary stability during placement more than any other factors. Primary stability itself is mainly influenced by surgeon`s experience in the proper following of surgical protocol.

Objectives: Familial Adenomatous Polyposis (FAP) is an autosomal dominant predisposition to colon cancer.This hereditary genetic disease is characterized by more than 100 adenomatous polyps in colon and rectum.Additional features may include desmoids tumors, polyps in the upper gastrointestinal tract, osteomas and Congenital Hypertrophy of the Retinal Pigment Epithelium (CHRPE). A mutation in APC (Adenomatous Polyposis Coli) is found in the majority of cases. Mutation detection and genetic analysis of APC in this syndrome is highly recommended as the penetrance is almost 100% by 40 years of age. The APC gene expanding on 5q21- q22 has 15exons and has an ORF with 8538 nucleotides which codes a protein with 2843 amino acids.Materials and Methods: 5 families among 150 families were selected according to accepted diagnosis criteria of FAP. CSGE (Conformation Sensitive Gel Electrophoresis) technique for the first time was set up to screen mutations in all 15 exons of APC gene by this technique. Direct sequencing was used as gold standard to confirm CSGE results.Results: CSGE analysis showed electrophoresis migration anomalies and heteroduplex formation in exon 15 of APC in all patients. Further analysis by direct sequencing characterized these heteroduplexes as deleterious mutations. These mutations can be classified as non sense, frame shift and deletion.Conclusion: For the first time, CSGE technique was set up for mutation screening of coding and some parts of non coding regions of APC. In comparison with other screening methods, this technique has many advantages so it can be used in routine clinical laboratories. As mutation detection in APC is laborious, needs high tech technology and is expensive, finding Sensitive and cost effective mutation screening technique would have direct positive effect on clinical management of families with familial susceptibility to colon cancer.

In this paper, three solutions for scheduling problem of the Single-Load and Multi-Load Automated Guided Vehicles (AGVs) in Container Terminals are proposed. The problem is formulated as Constraint Satisfaction and Optimization. When capacity of the vehicles is one container, the problem is a minimum cost flow model. This model is solved by the highest performance Algorithm, i.e. Network Simplex Algorithm (NSA). If the capacity of the AGVs increases, the problem is a NP-hard problem. This problem has a huge search space and is tackled by the Simulated Annealing Method (SAM). Three approaches for its initial solution and a neighborhood function to the search method are implemented. The third solution is a hybrid of SAM and NSA. This hybrid is applied to the Heterogeneous AGVs scheduling problem in container terminals. Several the same random problems are generated, solved by SAM with the proposed approaches and the simulation results are compared. The experimental results show that NSA provides a good initial solution for SAM when the capacity of AGVs is heterogeneous.